DNA Extraction, Library Preparation, and Sequencing
Course Overview
This module provides comprehensive training in molecular workflows, from DNA extraction to library preparation and sequencing using Oxford Nanopore Technologies (ONT) and Illumina platforms. Participants will be equipped to generate genomic data crucial for AMR research, outbreak tracking, and epidemiological studies.
Oxford Nanopore Technologies (ONT) Sequencing
Includes ONT-focused library preparation, flow-cell operation, and QC workflow. Prior exposure to basic molecular biology techniques required.
Learning Objectives
- ✅Extract high-quality DNA suitable for long-read sequencing
- ✅Prepare sequencing libraries and perform runs on Nanopore devices
- ✅Conduct base-calling, demultiplexing, and quality control of sequence data
- ✅Understand applications of Nanopore sequencing in AMR detection and genomic surveillance
Course Content
- 📘Overview of next-generation sequencing technologies
- 📘DNA extraction methods and quality assessment
- 📘Library preparation, barcoding, and flow-cell operation
- 📘Base-calling and sequence data quality control
- 📘Introduction to assembly and annotation of bacterial genomes
- 📘Linking genomic results to AMR detection and typing and application of genomic data
Cost: $725
Additional Information
Maximum of 20 participants. Fee covers reagents, consumables, and training materials including manuals, SOPs, and stationery. Travel arrangements, accommodation and food not included.
Illumina Sequencing
Learning Objectives
- ✅Extract and quantify high-quality DNA suitable for Illumina sequencing workflows
- ✅Prepare libraries for short read sequencing
- ✅Understand the sequencing-by-synthesis (SBS) principle and how it differs from long-read platforms
- ✅Perform initial data handling including demultiplexing, base-calling, and quality assessment using bioinformatics pipelines
- ✅Apply Illumina sequencing data to microbial genomics, AMR surveillance, and epidemiological investigations
Course Content
- 📘Overview of Illumina sequencing technology and applications in AMR and microbial genomics
- 📘DNA extraction methods for bacteria and sample quality evaluation
- 📘Illumina library preparation workflow
- 📘Quality control of libraries using Bioanalyzer
- 📘Flow cell loading, run setup, and sequencing instrument operation
- 📘Data generation, monitoring, and output management
- 📘Introduction to sequencing data storage
- 📘Troubleshooting and quality assurance throughout the workflow
Cost: $823
Additional Information
Maximum of 20 participants. Fee covers reagents, consumables, and training materials including manuals, SOPs, and stationery. Travel arrangements, accommodation and food not included.
